Mammalian Cell Expression Services
Mammalian cell is an important expression system in the gene engineering pharmaceutical research. It has highly sophisticated mechanism of protein folding and post-translational modification, the recombinantly expressed protein has correct folding and modification, thus the conformation is much closer to human. Because the product is nearest to the natural protein molecule of higher organisms in molecular structure, physical and chemical properties and biological function, mammalian cell has become the preferred host for producing therapeutic recombinant proteins.
BioMetas provide protein production by transient expression in both HEK293 and CHO cell lines. We are able to carry out high throughput and high yield production for recombinant antibodies and proteins. The transiently expressed products can be applied to antigen generation, antibody discovery, protein structure and function research, etc.
- Highlights
- From transient to stable protein expression
- Proprietary mammalian high performance system
- Diverse purification methods, e.g. affinity chromatography, SEC, IEX, HIC, etc.
- Short turnaround time and competitive price
Production Process
Case Study
Addition of an affinity tag is a useful method for purifying a target protein from the total cell lysate. In general fusion- protein tags can be classified into two main groups: solubility tags (GST, MBP, NusA, Trx and SUMO) and affinity tags (Poly His, Flag, HA, Strep II). And tags can be place on protein’s C-terminus or N-terminus. Below is a case study where we tested two tags (MBP and flag) on 12 proteins to investigate their impact on protein’s yield and quality.