Cancer Cell Biology
Cancer Cell Biology
- Cell line characterization
- Cas9 stable cell line generation
- Library virus packaging and tittering
- Library screen
- NGS (with partner)
- ~600 cell lines banked
- ~100 cell lines coming soon
- STR verified and mycoplasma tested
- Stringent data QC
- Flexible data reporting format
Interested in our Services?
- siRNA‐Mediated Gene Knockdown
- shRNA‐Mediated Gene Knockdown
- CRISPR‐based Gene Knockout/Knockdown
- Cell line characterization
- Cas9 stable cell line generation
- Library virus packaging and tittering
- Library screen
- NGS (with partner)
- ~600 cell lines banked
- ~100 cell lines coming soon
- STR verified and mycoplasma tested
- Stringent data QC
- Flexible data reporting format
- siRNA‐Mediated Gene Knockdown
- shRNA‐Mediated Gene Knockdown
- CRISPR‐based Gene Knockout/Knockdown
CRISPR Library Screening
- Highlights
- Two-vector System
• Cas9 stable cell line generation and Characterization
• CRISPR library screen
- Size from 15,000 to 80,000
- With or without fluorescence marker
- Library screen with or without compounds treatment
- Single-vector System
- Small library size
- With or without fluorescence marker
- Library screen with or without compounds treatment
CRISPR Library Screening
- Highlights
- Two-vector system
• Cas9 stable cell line generation and Characterization
• CRISPR library screen
- Size from 15,000 to 80,000
- With or without fluorescence marker
- Library screen with or without compounds treatment
- Single-vector system
- Small library size
- With or without fluorescence marker
- Library screen with or without compounds treatment
- Services
- ~800 cell lines banked, ~100 cell lines coming soon
- Well Characterized
- Verified by STR analysis
- PCR to prove mycoplasma free.
- Cell morphology
- Cell growth curves/seeding density and doubling time determined
- Multiple assay readout options
- CellTiter Glo assay
- CyQUANT® Direct Cell Proliferation Assay
- Caspase-Glo®3/7 Assay
- Other assay readouts
- Stringent QC system
- Well-trained and experienced team
- Small library size
- With or without fluorescence marker
- Library screen with or without compounds treatment
Cancer Cell Line Panel Screening
Well-Preserved Purchasing Records
Images Taken at Early Passage
HCC1143
MDA-MB-175-VII
- Highlights
- 96-well plates or 384-well plates
- 6-well plates or 6-well plates can be used for 14-day 2D clonogenic assay
- Short term (3 to 6 days) or long term proliferation assay (7 to 14 days)
- Single drug or combination drug testing
- Performed on a regular basis
- Customized panel, flexible size
- Fast turnaround time (Expedited scheduling can be arranged prior to study commencement.)
- Highly reproducible data
- Cost-savings
- Experienced, responsive and dedicated scientist team
Cancer Cell Line Panel Screening
- Services
- ~600 cell lines banked, ~100 cell lines coming soon
- Well Characterized
- Verified by STR analysis
- PCR to prove mycoplasma free.
- Cell morphology
- Cell growth curves/seeding density and doubling time determined
- Multiple assay readout options
- CellTiter Glo assay
- CyQUANT® Direct Cell Proliferation Assay
- Caspase-Glo®3/7 Assay
- Other assay readouts
- Stringent QC system
- Well-trained and experienced team
- Small library size
- With or without fluorescence marker
- Library screen with or without compounds treatment
Well-Preserved Purchasing Records
Images Taken at Early Passage
HCC1143
MDA-MB-175-VII
- Highlights
- 96-well plates or 384-well plates
- 6-well plates or 6-well plates can be used for 14-day 2D clonogenic assay
- Short term (3 to 6 days) or long term proliferation assay (7 to 14 days)
- Single drug or combination drug testing
- Performed on a regular basis
- Customized panel, flexible size
- Fast turnaround time (Expedited scheduling can be arranged prior to study commencement.)
- Highly reproducible data
- Cost-savings
- Experienced, responsive and dedicated scientist team
Target Validation
- Highlights
- siRNA/shRNA or CRISPR-based target gene Knockdown
- shRNA/sgRNA can be provided by client or generated at BioMetas
- Taqman or Western blot analysis to confirm the knockdown efficiency
- Functional assays to evaluate the phenotypes
- CRISPR-based target gene Knockout
- Single-vector system or two-vector system
- Functional assays to evaluate the phenotypes
Case Study Samples
Cas9 expression by FACS
KO determined by Western Blot
Cell growth after gene KO by 14-day 2D clonogenic assay
KD efficiency determined by Western Blot and RT-qPCR (Taqman)
Cell growth after CRISPR-based target gene KD by 14-day 2D clonogenic assay
Target Validation
- Highlights
- siRNA/shRNA or CRISPR-based target gene Knockdown
- shRNA/sgRNA can be provided by client or generated at BioMetas
- Taqman or Western blot analysis to confirm the knockdown efficiency
- Functional assays to evaluate the phenotypes
- CRISPR-based target gene Knockout
- Single-vector system or two-vector system
- Functional assays to evaluate the phenotypes
Cas9 expression by FACS
KO determined by Western Blot
Cell growth after gene KO by 14-day 2D clonogenic assay
KD efficiency determined by Western Blot and RT-qPCR (Taqman)
Cell growth after CRISPR-based target gene KD by 14-day 2D clonogenic assay
- Services
- Cell viability/anti-proliferation/apoptosis assay/cytotoxicity assay
• 2D assays (single agents or combinations)
- CellTiter Glo assay/CellTiter-Blue assay (3-7 day in 96-well or 384-well plate, 14-day long term proliferation assay)
- 2D CyQUANT Direct Cell Proliferation Assay (3-7 day in 96-well or 384-well plate)
- 2D Cell viability assay by direct cell counting (3-7 day in 96-well or 384-well plate, 14-day long term proliferation assay)
- 2D clonogenic assay in 6-well or 12-well plates (10-14 days)
- Caspase-Glo 3/7 Assay
- CytoTox-Glo Cytotoxicity Assay (up to 24 hours)
• 3D assays (single agents or combinations)
- 3D growth in Ultra-Low Attachment plates (5-10 days, 96-well plates)
- 3D Matrigel assay (5-14 days, 96-well plates)
- Soft agar assay (10-14 days, 96-well plates)
- Functional assays by FCM (cell cycle analysis, apoptosis assay)
- Cell signaling assays by Western blot, qRT-PCR, high-content Imaging or reporter assay
Cell-Based Assays
14-day Long Term Proliferation Assay
- Cells are seeded into 96-well plates and treated with compounds for 4 days.
- Aspirate an aliquot of cells and load with Calcein AM.
- Count the cells with Cytation 5. Split the master plate and re-plate the cells at the original seeding density. Count and re-plate the cells on day 3, day 7, day 11 and get a final counting on day 14.
Cell-Based Assays
- Services
- Cell viability/anti-proliferation/apoptosis assay/cytotoxicity assay
• 2D assays (single agents or combinations)
- CellTiter Glo assay/CellTiter-Blue assay (3-7 day in 96-well or 384-well plate, 14-day long term proliferation assay)
- 2D CyQUANT Direct Cell Proliferation Assay (3-7 day in 96-well or 384-well plate)
- 2D Cell viability assay by direct cell counting (3-7 day in 96-well or 384-well plate, 14-day long term proliferation assay)
- 2D clonogenic assay in 6-well or 12-well plates (10-14 days)
- Caspase-Glo 3/7 Assay
- CytoTox-Glo Cytotoxicity Assay (up to 24 hours)
• 3D assays (single agents or combinations)
- 3D growth in Ultra-Low Attachment plates (5-10 days, 96-well plates)
- 3D Matrigel assay (5-14 days, 96-well plates)
- Soft agar assay (10-14 days, 96-well plates)
- Functional assays by FCM (cell cycle analysis, apoptosis assay)
- Cell signaling assays by Western blot, qRT-PCR, high-content Imaging or reporter assay
14-day Long Term Proliferation Assay
- Cells are seeded into 96-well plates and treated with compounds for 4 days.
- Aspirate an aliquot of cells and load with Calcein AM.
- Count the cells with Cytation 5. Split the master plate and re-plate the cells at the original seeding density. Count and re-plate the cells on day 3, day 7, day 11 and get a final counting on day 14.
14-day Long Term Proliferation Assay
- The readout can be adjusted to direct cell counting.
- The assay duration can be extended to 21 days.
14-day Long Term Proliferation Assay
- The readout can be adjusted to direct cell counting.
- The assay duration can be extended to 21 days.
Apoptosis Assay
- Caspase 3/7 Glo assay is relative high throughput but multiple timepoints should be considered. 96-well plates are preferred.
- Apoptosis analysis by FCM can be run in 24-well plates to 6-well plates.
Caspase 3/7 Glo assay is relative high throughput but multiple timepoints should be considered. 96-well plates are preferred.
Apoptosis analysis by FCM can be run in 24-well plates to 6-well plates.
Apoptosis Assay
- Caspase 3/7 Glo assay is relative high throughput but multiple timepoints should be considered. 96-well plates are preferred.
- Apoptosis analysis by FCM can be run in 24-well plates to 6-well plates.
Cell Cycle Analysis by Flow Cytometry
Cell cycle analysis can be run in 12-well or 6-well plates
Cell cycle analysis can be run with or without Edu incorporation.
Cell Cycle Analysis by Flow Cytometry
- Cell cycle analysis can be run in 12-well or 6-well plates
- Cell cycle analysis can be run with or without Edu incorporation.
NanoBRET Protein: Protein Interaction
NanoBRET Protein: Protein Interaction
High Content Imaging
Stress Granule induced by Sodium Arsenite in U2OS cells.
Cells were stained with G3BP1 primary antibody and Alexa Fluor 488 conjugated secondary antibody.
DAPI was used for nuclear staining
The plate was scanned using Cytation 5 (60x objective)
High Content Imaging
Stress Granule induced by Sodium Arsenite in U2OS cells.
Cells were stained with G3BP1 primary antibody and Alexa Fluor 488 conjugated secondary antibody.
DAPI was used for nuclear staining
The plate was scanned using Cytation 5 (60x objective)
Cell Signaling Assays
- Highlights
- Protein phosphorylation
- AlphaLISA
- HTRF
- Western blot
- Nuclear translocation by high content imaging
- Reporter assay
- RT-qPCR to determine the downstream gene expression
PD marker analysis via Western blot, RT-qPCR, or FCM
Cell Signaling Assays
- Highlights
- Protein phosphorylation
- AlphaLISA
- HTRF
- Western blot
- Nuclear translocation by high content imaging
- Reporter assay
- RT-qPCR to determine the downstream gene expression
PD marker analysis via Western blot, RT-qPCR, or FCM
- Highlights
- Strong team with extensive experiences in stable cell line generation for research purposes
- 12 senior scientists with >9 years’ experience in stable cell line generation
- >1000 stable cell lines have been generated by the team (overexpression, knockdown, knockout)
- Extensive experience in gene knockout/knockdown using CRISR/Cas9/dCas9 Technology
- Large selection of vectors
- Robust selection of cell-lines
- ~600 human cell lines
- 14 non-human cell lines (including CHO-K1, 4T1 etc.)
- Multiple assays can be chosen for stable cell line characterization
- All the stable cell lines will be verified by STR analysis and PCR will be run for mycoplasma test
Stable Cell Line Generation
Stable Cell Generation Timeline
Stable Cell Line Generation
- Highlights
- Strong team with extensive experiences in stable cell line generation for research purposes
- 12 senior scientists with >9 years’ experience in stable cell line generation
- >1000 stable cell lines have been generated by the team (overexpression, knockdown, knockout)
- Extensive experience in gene knockout/knockdown using CRISR/Cas9/dCas9 Technology
- Large selection of vectors
- Robust selection of cell-lines
- ~600 human cell lines
- 14 non-human cell lines (including CHO-K1, 4T1 etc.)
- Multiple assays can be chosen for stable cell line characterization
- All the stable cell lines will be verified by STR analysis and PCR will be run for mycoplasma test
Stable Cell Generation Timeline
Stable Cell Generation Workflow
